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RPA phosphorylation induced by telomeric damage is dependent on Mec1p. Western blot analysis of Rfa1p and Rfa2p from cdc13 cells harvested immediately before and after incubation at the restrictive temperature for 2 h. Strains employed were: TWY146 (cdc13), TWY158 (cdc13 mec1-1), TWY149 (cdc13 mec1-2) and TWY150 (cdc13 mec1-3). P-Rfa2p, phosphorylated form of Rfa2p.
