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. 2000 Oct 1;28(19):3725–3732. doi: 10.1093/nar/28.19.3725

Figure 5.

Figure 5

Rfa1p and Rfa2p phosphorylation induced by telomeric damage exhibit different genetic dependencies. (A) Western blot analysis of RPA from cells harvested immediately before and after incubation at the restrictive temperature for 2 h. Strains employed were DLY408 (cdc13), DLY409 (cdc13 rad9), DLY410 (cdc13 rad24), DLY411 (cdc13 rad9 rad24), DLY418 (- - -) and DLY419 (rad9). These strains contain a mutant allele of CDC15 that allows for mitotic arrest of checkpoint-deficient strains at the restrictive temperature (40). (B) Western blot analysis of Rfa2p from DLY409 cells incubated at the restrictive temperature for 1 h. (C) Phosphatase treatment of native extract from DLY409 cells incubated at the restrictive temperature for 2 h.