Table 1. Strains used in this study.

Strain	Genotype	Reference
TWY397	MATa ura3 his7 leu2 trp1	28
TWY308	MATα mec1-1 ura3 trp1	28
TWY312	MATa mec2-1 ura3 his7 trp1	28
TWY316	MATa mec3-1 ura3 his3 trp1	28
TWY398	MATa rad9Δ::LEU2 ura3 his7 leu2 trp1	28
TWY146	MATα cdc13-1 ura3 his7	28
TWY158	MATα mec1-1 cdc13-1 ura3 his7	28
TWY149	MATα mec1-2 cdc13-1 ura3 his7	28
TWY150	MATα mec1-3 cdc13-1 ura3 his7	28
DLY285	MATa mec1-1::HIS3 ura3 his3 leu2 trp1	31
YDM937	MATa mec1-1 tel1Δ1::HIS3 ura3-52 his3Δ200 leu2Δ1 lys2-801 trp1Δ1	33
DLY408a	MATa cdc13-1 cdc15-2	40
DLY409	MATa cdc13-1 cdc15-2 rad9::HIS3	40
DLY410	MATa cdc13-1 cdc15-2 rad24::TRP1	40
DLY411	MATa cdc13-1 cdc15-2 rad9::HIS3 rad24::TRP1	40
DLY418	MATa cdc15-2	40
DLY419	MATa cdc15-2 rad9::HIS3	40
W303-1A	MATa ade2-1 can1-100 his3-11,15 leu2-3,112 trp1-1 ura3-1	61

^aDLY strains 408–411, 418 and 419 are congenic and have the following additional genotype: bar1::hisG ade2-1 can1-100 ura3 leu2-3,112 his3-11 trp1-1.