Fig. 2. PNUTS is necessary for normal angiogenic sprouting.
a In vitro sprouting was analyzed under VEGF (50 ng/ml) stimulation. Representative images are shown, red arrows indicate discontinuous sprouts. b Quantification of cumulative sprout length (left) and number of discontinuous sprouts (right) (n = 30 spheroids per group in 3 independent experiments were measured). c Endothelial Pnuts expression of PNUTSEC-KO and WT mice was assessed by RT-qPCR in intima samples 15 days after initiating tamoxifen treatment and normalized to Rplp0 mRNA (n = 3). d In vivo angiogenesis was tested by aortic ring assay in WT and PNUTSEC-KO mice in basal conditions or upon 48 h of VEGF (10 ng/ml) stimulation. e Quantification of aortic ring sprouting. n = 4–5 mice per group. *p < 0.05, **p < 0.01, ***p < 0.001. Error bars depict the standard error of the mean (SEM).