Table 1.
Primers used in this study
| Use | Gene | Forward primer (5ʹ-3ʹ) | Reverse primer (5ʹ-3ʹ) |
|---|---|---|---|
| qPCR | Human PNUTS | GGGTTCGGGTCCCATAGACCCC | GCCGCCAACGTCAATAAATTTGAC |
| Human p21 | AGTCAGTTCCTTGTGGAGCC | CATTAGCGCATCACAGTCGC | |
| Human RPSA | ATAAGGACGTCATTTCCTGCC | TTTAAGTTACGACGGGAATCCAGAA | |
| Human RPLP0 | TCGACAATGGCAGCATCTAC | ATCCGTCTCCACAGACAAGG | |
| Human SEMA3B | TGTGCTCCGGAGACTCGT | CGCTGGAAAGTCCACTCCAC | |
| Mouse Pnuts | TGGGCAGTGGACATCGTTCTCA | GGCGGTTTGACATGTCTCCTCCA | |
| Mouse Sema3b | CACCTCCAGTGGTGTCTTCC | AAAGGTCCCAAGAAGGCTCG | |
|
Mouse Rplp0 |
GCGTCCTGGCATTGTCTGT | GAAGGCCTTGACCTTTTCAGTAA | |
| Mutagenesis | PNUTS | TGGATGAAACAGAGCGGGTAAATGTGA | TCACATTTACCCGCTCCGTTTCATCCA |
| Genotyping | Cre | GCGGTCTGGCAGTAAAAACTATC | GTGAAACAGCATTGCTGTCACTT |
| Cpxm1 | ACTGGGATCTTCGAACTCTTTGGAC | GATGTTGGGGCACTGCTCATTCACC | |
| PNUTSfl | CATGACAATATGGAGGAGAAAGTGCCTTG | AGGAGAAAGAAGAGAACAGGGGACCATAAT |