Figure 9.

Mutation discrimination with multiplex and singleplex reactions for ΔF508. Both the mutant and normal Scorpions are able to differentiate between samples with or without mutant DNA in the single- and multiplex reactions. N, normal sample; H, heterozygous sample; Neg, negative control; sw, singleplex reaction with the normal Scorpion; sm, singleplex reaction with the mutant Scorpion; mu, multiplex reaction. The baseline is proportionally adjusted in this case as although there is sample–sample variation in baseline this is mainly between samples containing different Scorpions. Importantly there is little variation between samples with the same Scorpion. (A) Fluorescence detection on channel 1. This channel detects the normal Scorpion labelled with FAM. (B) Fluorescence detection on channel 2. This channel detects the mutant Scorpion labelled with ROX and an internal FAM.