Table 2.

Percentage of total MDSCs and MDSC subpopulations from PBMCs via flow cytometry.

MDSC Subpopulation	Healthy Subjects (n=6)	Sepsis Day 4 ± 1 (n=7)	Sepsis Days 14-21 (n=3)
% Total MDSCs	15.1 (8.1, 16.6)	39.2 (25.2, 55.6)	44.8 (35.6, 56.3)
% E-MDSC	68.1 (49.8, 78.7)	1.4 (0.7, 5.9)	2.3 (2.2, 9.0)
% PMN-MDSC	14.6 (7.4, 36.9)	79.5 (64.4, 89.0)	80.7 (52.0, 85.1)
% M-MDSC	9.2 (4.9, 12.7)	9.1 (8.1, 12.7)	11.5 (9.7, 35.9)
% Ungated	2.0 (1.4, 3.2)	0.7 (0.5, 1.3)	0.7 (0.7, 3.1)

Percentages of MDSC populations in representative septic patients. Blood was collected from healthy subjects (n=6), day 4 ± 1 septic patients (n=7), and late sepsis patients at days 14-21 (n=3)). PBMCs were isolated and prepared for flow cytometry. Viable cells determined followed by gating of CD11b⁺ and CD33⁺ cells. HLA-DR^low cells selected to capture total MDSCs. CD11b⁺ CD33⁺ HLA-DR^low cells outside the gating of the three MDSC subpopulations are classified as “% Ungated.” Results reported as median (Q1, Q3). E, early; PMN, granulocytic; M, monocytic.