Figure 2.

Phagocytosis of IgG-opsonized latex beads in PEMϕs expressing Ly49Q-WT or Ly49Q-YF. (A) The numbers of 6-µm-diameter phagocytosed beads were counted. Statistical analyses were conducted using Mann–Whitney U test. (B) Immunohistochemical analyses of actin structures in PEMϕs soon after the addition and centrifugation of fluorescence-labeled beads (ϕ2 µm). Arrow heads indicate the sites where beads bound and actin polymerized. White boxes in the left photographs indicate the areas enlarged in the right photographs. Scale bar; 10 µm. (C) Localization of Ly49Q visualized by anti-Flag staining. PEMϕ prepared from Ly49Q-WT or Ly49Q-YF Tg mice were fixed soon after the addition and centrifugation of fluorescence-labeled beads (ϕ2 µm) and stained with the reagent indicated. (D, E) Distribution of MHC-I (D) and β2 integrin (E) in PEMϕ after capturing latex beads (ϕ2 µm). Lower pictures in (E) show serial sections of Z-planes of the PEMϕs stained with anti-β2 integrin-antibody shown in the upper images. (F) F-actin distribution in PEMϕs 60 min after bead addition. Serial sections of the Z-plane of F-actin-visualized PEMϕs are shown on the left. Histograms on the right show the profiles of signal intensities along the dashed lines in the photographs. All results shown are representative of at least two independent experiments.