Figure 2.
Quantitative analysis of aminoacyl-tRNAIle in cells. Total RNA was isolated from cybrid cells under acidic conditions as described in Materials and Methods. The same amount of each RNA sample was loaded onto an acidic polyacrylamide gel and the aminoacyl-tRNAIle and uncharged tRNAIle were separated, followed by northern hybridization. Upper and lower bands correspond to aminoacyl- and uncharged tRNAIles, respectively; lanes 1, 2 and 3, alkali-treated CM1-9 RNA and RNA samples from the wild-type cybrid clone CM1-9 and from the mutant cybrid clone CM114-5, respectively.