FIGURE 5.

Inhibitory impacts of standard chemotherapy and the most active nanocomplex on the self-renewal potential of human and mouse glioma stem cells (GSCs) and radioresistance GSCs-RR. (A) Inhibition potency of temozolomide (TMZ) and diethyldithiocarbamate (DE)-FeO NPs on sphere formation of human MGG18, mouse GS, MGG18-RR, and GSRR as indicated by (I) dose response curves and (II) half maximal inhibitory concentration (IC₅₀) values as well as (B) morphological variations of the formed spheres using (I,II) phase contrast microscopy and (III) fluorescence microscopy for mCherry-labeled GS and GSRR. (C) A decrease in (I,II) counts and (III,IV) area of the formed treated spheres (MGG18, GS, MGG18-RR, and GSRR, respectively) relative to the untreated control. Data are demonstrated as mean ± SEM. DE-FeO NPs nanocomplex was compared to TMZ. Values are considered statistically significant at p < 0.05*, <0.01**, and <0.001***.