FIGURE 6.

Suppressive effects of standard chemotherapy and the most active nanocomplex on cancer repopulation and therapeutic resistance potentials of human and mouse glioma stem cells (GSCs) and radioresistance GSCs-RR. (A) Growth inhibition effect of temozolomide (TMZ) and diethyldithiocarbamate (DE)-FeO NPs on the attached differentiated glioma cells of human MGG18 and mouse GS in terms of (I) dose response curves and (II) half maximal inhibitory concentration (IC₅₀) values, as well as (III) morphological variations in the treated differentiated cancer cells using phase contrast microscopy (Magnification ×200, scale bar 100 µm). Data are demonstrated as mean ± SEM. DE-FeO NPs nanocomplex was compared to TMZ. Values are considered statistically significant at p < 0.05*, <0.01**, and <0.001***. (B) Relative fold increment in the chemosensitivity and radiosensitivity response of pretreated MGG18-RR and GSRR with DE-FeO NPs to (I) TMZ and (II,III) radiation (2Gy and 5Gy, respectively), as well as (C) their morphology (I) human spheres using phase contrast microscopy (Magnification ×200, scale bar 100 µm) and mouse spheres using (II) phase contrast microscopy and (III) Fluorescence microscopy (magnification ×200, scale bar 100 µm).