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. 2024 Apr 1;65(5):100540. doi: 10.1016/j.jlr.2024.100540

Fig. 10.

Fig. 10

DGAT inhibition promotes retention of nLD- and LAPS-associated proteins on the INM of Huh7 cells. Huh7 cells were treated with oleate or oleate plus iDAGT1 and iDGAT2 as described in Fig. 9. A: Huh7 cells transiently expressing Lipin1α-V5 were immunostained with V5 and PML primary and AlexaFluor-555 and AlexaFluor-647 secondary antibodies, respectively. LDs were visualized with BODIPY493/503 (bar, 5 μm). B: the freqency of cells positive for PML patches was quantified after treatment with oleate or oleate plus DGATi for the indicated times using 15–18 fields of cells (30–60 cells/field) from two experiments. C: Huh7 cells transiently expressing a GFP-DAG biosensor were immunostained with a CCTα primary and AlexaFluor-555 secondary antibodies (bar, 5 μm). D: Huh7 cells were immunostained with CCTα and PML primary followed by AlexaFluor-555 and AlexaFluor-647 secondary antibodies. LDs were visualized with BODIPY 493/503 (bar, 5 μm). CCTα, CTP:phosphocholine cytidylyltransferase; INM, inner nuclear membrane; LAPS, lipid-associated promyelocytic leukemia structure; nLD, nuclear lipid droplet; PML, promyelocytic leukemia.