Fig. 3.

Differentiated Caco2 cells contain a constitutive pool of large nLDs. A: representative confocal image of differentiated Caco2 and CCTα KO cells that received no addition (NA) or oleate (500 μM) for 24 h used for quantitation in panels B–G (bar, 5 μm). Cells were immunostained with PML primary and AlexaFluor-555 secondary antibodies. LDs were visualized with BODIPY 493/503. B: quantification of cLDs per cell. C: mean area of cLDs. D: quantitation of nLDs per cell. E: mean area of nLDs. F: ratio of nLDs to total cLDs plus nLDs. G: percentage of LAPS (PML+ve nLDs) in oleate-treated cells. Results are presented as scatter plots showing the mean and SD from 15-24 fields of cells (60–100 cells/field) from four separate experiments. Significance compared to matched control Caco2 cells was determined by two-way ANOVA. ∗∗∗P < 0.001, ∗∗P < 0.01, ∗P < 0.05. CCTα, CTP:phosphocholine cytidylyltransferase; cLD, cytoplasmic lipid droplet; LAPS, lipid-associated promyelocytic leukemia structure; nLD, nuclear lipid droplet; PML, promyelocytic leukemia.