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. 2024 Apr 1;65(5):100540. doi: 10.1016/j.jlr.2024.100540

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© 2024 The Authors

This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).

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Fig. 5 — nLDs and LAPS in Caco2 cells do not harbor apoCIII or apoAI. A and B: Huh7 and control undifferentiated (Ct) and differentiated (Df) Caco2 cells were immunostained with antibodies against apoAI (panel A) or apoCIII (panel B) and PML, followed by AlexaFluor-555 and AlexaFluor-647 secondary antibodies. LDs were visualized with BODIPY 493/503 and imaged by confocal microscopy (bar, 10 μm). ApoAI- and apoCIII-positive LAPS are indicated by arrows in the Huh7 cell panels. LAPS, lipid-associated promyelocytic leukemia structure; nLD, nuclear lipid droplet; PML, promyelocytic leukemia.