Fig. 7.

Caco2 cells contain a pool of pre-existing nLDs and LAPS. A and B: Caco2 cells were treated with [³H]oleate (100 μM) and iDGAT1 (10 μM), iDGAT2 (10 μM), or both inhibitors (10 μM each) for 4 h. [³H]Oleate incorporation into TAG (panel A) and cholesteryl ester (CE, panel B) was measured as described in Materials and Methods. The results are the mean and SD of two experiments carried out in duplicate. C–G: Caco2 cells were treated with oleate (500 μM, •) or oleate plus DGATi (10 μM, ▪). At the indicated times, the nLD/cell (panel C), LAPS/cell (D), CCTα-positive nLDs (panel E), average nLD cross-sectional area (panel F), average cLD cross-sectional (G) were quantified from 15-18 fields of cells (30–60 cells/field) from two experiments. CCTα, CTP:phosphocholine cytidylyltransferase; cLD, cytoplasmic lipid droplet; LAPS, lipid-associated promyelocytic leukemia structure; nLD, nuclear lipid droplet; TAG, triglyceride.