Fig. 8.

nLDs and LAPS in Caco2 cells associate with the nuclear envelope and nucleoplasmic reticulum. A and B: undifferentiated Caco2 cells treated with oleate (500 μM) for 2 h were fixed and immunostained with emerin and PML primary and AlexaFluor-555 and AlexaFluor-647 secondary antibodies, respectively. Z-stacks of cells containing nLDs and LAPS are shown in panels A (bar, 2 μm). 3D rendering of these projections (panels B) are described in Materials and methods. The 3D images are viewed from the top surface and rotated 90^° relative to the x-y plane in panel A. C and D: differentiated Caco2 cells treated with oleate (500 μM) for 24 h were immunostained with LMNA/C and PML primary and AlexaFluor-555 and AlexaFluor-647 secondary antibodies, respectively. Z-stacks in panel C (bar, 2 μm) and 3D images in panel D (rotated slightly left and viewed from the basolateral surface) were generated as described above. LAPS, lipid-associated promyelocytic leukemia structure; nLD, nuclear lipid droplet; PML, promyelocytic leukemia.