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. 2024 Feb 10;52(8):4151–4166. doi: 10.1093/nar/gkae069

Figure 3.

Figure 3.

Inhibition of Pol II by CDKis prevents its degradation. (A) Schematic representation of Pol II transcription cycle and effect of the transcription inhibitors. THZ1 inhibits the phosphorylation of Ser5 by Cdk7. Flavopiridol inhibits the phosphorylation of Ser2 by Cdk9. (B) Immunoblot of whole-cell and subcellular fractions of U2OS cells treated with CDK inhibitors THZ1 and Flavopiridol for 90 min and then co-incubated with BMH-21 1 μM for 3 additional hours. (C) Immunoblot analysis of whole-cell lysates using of U2OS cells treated with CDK inhibitors as in (B) and co-incubated with CBL0137 (1 μM), Aclarubicin (1 μM) and Actinomycin D (200 nM).