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. 2024 Apr 4;52(8):4456–4465. doi: 10.1093/nar/gkae223

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© The Author(s) 2024. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial License (https://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com

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Figure 4. — Distribution of DNA and Dps molecules along doubly-tethered DNA. (A) Overlaid fluorescence signals obtained from DNA labeled with SYTOX orange (green) and Cy5-labeled Dps (magenta) after the formation of Dps–DNA complexes and the cessation of flow. A representative example of a stretched DNA molecule with a Dps–DNA complex is highlighted (cyan rectangle). (B) Plot showing the intensity distribution of Cy5-Dps (magenta) along DNA labeled with SYTOX orange (green) obtained by averaging the intensity of Cy5 and SxO from 90 different structures. Inset represents the example molecule from (A), illustrating orientation of pixels used in the plot.