Table 2.
Summary of the equilibrium dissociation constants (Kd) for the binding of 5′-6FAM-tRNAArgCCU to A. thaliana KEOPS proteins as determined by microscale thermophoresis (MST). K, KAE1; B, BUD32; C, CGI121; P, PCC1; dimer, wild-type KEOPS complex (P2K2B2C2); monomer, KPR70D/R73D/A74EBC; mutant-1, Y36R/I37K/T38R/P39R/P40R/G41W/H42D; mutant-2, G43E/F44R/L45K/P46R/R47D/E48K; n.d., not determined
| KEOPS | K d (μM) | KAE1 mutants | K d (μM) | BUD32 mutants | K d (μM) |
|---|---|---|---|---|---|
| dimer | 18.08 ± 3.87 | KH117ABCP | 53.75 ± 17.70 | KBI47KCP | 221.59 ± 216.78 |
| monomer | 74.37 ± 13.51 | KD298RBCP | 97.90 ± 39.83 | KBK51ECP | 29.82 ± 10.66 |
| KBP | 37.99 ± 13.56 | KI17FBCP | 315.36 ± 16.72 | KBK55ECP | 104.29 ± 15.22 |
| KP | n.d. | KK202RBCP | 111.31 ± 21.71 | KBN58RCP | 9.81 ± 2.73 |
| BC | 61.58 ± 15.02 | KR284CBCP | 128.18 ± 5.68 | KBT162RCP | 10.47 ± 2.99 |
| CGI121 | n.d. | KA231GBCP | 138.89 ± 51.80 | KBS163RCP | 329.68 ± 11.10 |
| KBCPR73D | 352.7 ± 39.96 | KY305ABCP | 71.18 ± 13.80 | KBL165KCP | 302.42 ± 200.43 |
| – | – | Kmutant-1BCP | 5.27 ± 1.20 | KBR220stopCP | 148.39 ± 26.97 |
| – | – | Kmutant-2BCP | n.d. | KBR220ACP | 88.20 ± 15.58 |