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. 2024 Feb 27;52(8):4375–4392. doi: 10.1093/nar/gkae130

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© The Author(s) 2024. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 1. — COSA-1 interacts with MSH-5 and ZHP-3, but not with HIM-6. (A) Illustration of TurboID-mediated proximity labeling in cosa-1::3×HA::TurboID knock-in C. elegans strain. (B) Western blot analysis of expression of COSA-1::3×HA::TurboID fusion protein in vivo. (C) Colocalization of COSA-1::3xHA::TurboID with ZHP-3 at the CO-designated sites. Scalebar, 5 μm. (D) DAPI- stained oocyte from cosa-1::3×HA::TurboID knock-in strain. Scalebar, 5 μm. (E) Biotinylated MSH-5 proteins are detected by western blotting with antibodies against MSH-5. Proteins biotinylated by TurboID were enriched with streptavidin beads by affinity purification (AP) and examined by western blotting. (F) Analysis of interactions among pro-CO proteins by the yeast two-hybrid system.