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. 2024 Feb 27;52(8):4375–4392. doi: 10.1093/nar/gkae130

Figure 5.

Figure 5.

Formation of late CO-specific intermediates is compromised in cosa-1-4A mutant. (A) Representative SIM images of spread late pachytene nuclei of the indicated genotypes stained with antibodies against HA (green) to detect MSH-5 and HIM-3 (magenta). Scalebar, 1 μm. (B) Percentage of MSH-5 signals that are resolved as doublets at the CO-designated sites. Statistical analyses were conducted using t-test, ** P< 0.01. (C) Representative SIM images of recombination sites in late pachytene. Stainings for MSH-5, HIM-6, and HIM-3 are shown. (Scale bar, 500 nm). (D) Representative SIM images of SC bubbles (white arrowheads) surrounding MSH-5 foci in late pachytene nuclei. Stainings for MSH-5 and SYP-1 are shown (scale bar, 1 μm). (E) Quantification of formation of SC bubbles at the CO-designated sites in late pachytene nuclei for the indicated genotypes. Statistical analyses were conducted using t-test, ** P< 0.01, *** P< 0.005.