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. 2024 Feb 27;52(8):4375–4392. doi: 10.1093/nar/gkae130

Figure 6.

Figure 6.

Artificially tethering interaction-compromised COSA-1 to the CO-designated sites partially restores CO formation. (A) Representative images of late pachytene nuclei of the indicated genotypes stained with GFP booster to detect GFP::MSH-5 or ZHP-3::GFP (green), and antibodies against FLAG (magenta) to detect COSA-1, counterstained with DAPI (blue). Scalebar, 10 μm. (B) Representative images of diakinesis nuclei of the indicated genotypes stained with antibodies against HIM-3 (red), counterstained with DAPI (blue). Scalebar, 10 μm. White arrowheads indicate bivalents. (C) Quantitative analysis of the number of DAPI-stained bodies in diakinesis nuclei for the indicated genotypes. (D) Quantification of frequencies of male offspring among the progeny and (E) progeny viability of the indicated genotypes. Statistical analyses were conducted using t-test, n.s., not significant P> 0.05; * P< 0.05.