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. 2024 Feb 27;52(8):4375–4392. doi: 10.1093/nar/gkae130

Figure 7.

Figure 7.

Depletion of RTEL-1 partially restored bivalent formation in cosa-1-4A mutants. (A) Whole gonad immunostaining with antibodies against HA to detect RTEL-1 (green) and HIM-3 (magenta). Scalebar, 50 μm. (B) Representative images of nuclei from indicated regions of germline stained with antibodies against HA to detect RTEL-1 (green) and HIM-3 (magenta). Scalebar, 10 μm. (C) Quantification of the number of DAPI-stained bodies in diakinesis nuclei for the indicated genotypes. (D) Representative images of late pachytene nuclei from control and RTEL-1 depleted germline stained with antibodies against FLAG to detect COSA-1 (green) and HIM-3 (magenta), counterstained with DAPI (blue). Scalebar, 10 μm. (E) Quantification of the FLAG signal (COSA-1) in nuclei from control and RTEL-1 depleted germline. Statistical analyses were conducted using t-test, * P< 0.05.