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. 2000 Oct 1;28(19):3793–3800. doi: 10.1093/nar/28.19.3793

Figure 4.

Figure 4

A C-terminal truncated form of Ku80 is associated with mitochondrial DEB activity. A standard EMSA was performed (Materials and Methods) using HT1080 nuclear (N) or mitochondrial (M) protein extracts. Two micrograms of Ku80 Mab S10B1 (N-terminal antibody) was added to the EMSA in lanes 2 and 6. Ku80 Mab 111 (0.8 µg) (C-terminal antibody) was added to the EMSA in lanes 3 and 7. Linear competitor DNA was substituted for circular DNA in lanes 4 and 8. The large arrowhead indicates the mobility of nuclear DEB complex. The small arrowhead indicates the mobility of mitochondrial DEB complex.