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. 2024 May 8;7:0364. doi: 10.34133/research.0364

Fig. 3.

Fig. 3.

In vitro biofunctional evaluation of Oxa@HMI NPs on CT26 cells. Cellular uptake of RB@HM NPs (A), RB@HMI NPs (B), and RB@HMI NPs with inulinase (C) in CT26 cells analyzed by flow cytometry after incubation for 0, 2, 4, and 8 h, respectively (RB, Rhodamine B). (D) Cell viabilities of CT26 cells after 24 h incubated with various solutions at different concentrations (n = 5). (E) Representative CLSM images of intracellular O2 generation in CT26 cells after incubation with different solutions for 12 h under the hypoxic conditions. [Ru(dpp)3]Cl2 was used to indicate hypoxic levels. Scale bar: 10 μm. (F) Representative CLSM images of intracellular ROS generation in CT26 cells after incubation with different solutions for 24 h. DCFH-DA was used as ROS probe. Scale bar: 10 μm. (G) The CT26 cell viabilities evaluated by Live/Dead staining assay. Calcein AM: live cells. PI: dead cells. Scale bar: 10 μm. (H) Apoptosis of CT26 cell after different treatments analyzed by flow cytometry assay. The data are presented as mean ± SD.