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. 2024 Apr 29;20(7):2686–2697. doi: 10.7150/ijbs.92338

Figure 1.

Figure 1

BCA2 knockout results in a decreased proportion of BCSCs. (A) ALDH activity detected by ALDEFLUOR assay in HCC1806 cells with BCA2 knockout. Statistical results of the ALDH+ BCSC population in HCC1806 cells. (B) ALDH activity detected by ALDEFLUOR assay in HCC1937 cells with BCA2 knockout. Statistical results of the ALDH+ BCSC population in HCC1937 cells. (C) Protein expression levels of BCA2 in HCC1806 and HCC1937 cells with BCA2 knockout or their mock cells. (D) CD44+/CD24- BCSC population analysis in HCC1806 cells with BCA2 knockout. Statistical results of the CD44+/CD24- BCSC population in HCC1806 cells. (E) CD44+/CD24- BCSC population analysis in HCC1937 cells with BCA2 knockout. Statistical results of the CD44+/CD24- BCSC population in HCC1937 cells. (F) Mammosphere formation assay in HCC1937 cells with BCA2 knockout. Scale bar =250µM. Statistical results of the mamosphere formation ratio in HCC1937 cells. (G) The limited dilution assay was used to calculate the stem cell frequency in HCC1806 xenograft tumors. Statistical results of the tumorgenesis rate in HCC1806 cells. *, p < 0.05 **, p < 0.01