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. 2000 Oct 1;28(19):3695–3701. doi: 10.1093/nar/28.19.3695

Table 1. Purification of transcription elongation complexes from Physarum mitochondria.

  Total protein (mg) Units (U) Specific activity (U/mg) Fold Yield (%)
Isolated mito 50 750 15 1.6 147
Mito lysate 54.3 508.4 9.4 1 100
Lysate S130 29.3 463.1 15.8 1.7 91
Pooled TEC 1.7 885 520.6 55.4 174

One unit activity is defined as 1 µmol of UMP incorporated at 30°C in 30 min under standard assay conditions.

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