Table 1. Purification of transcription elongation complexes from Physarum mitochondria.
Total protein (mg) | Units (U) | Specific activity (U/mg) | Fold | Yield (%) | |
---|---|---|---|---|---|
Isolated mito | 50 | 750 | 15 | 1.6 | 147 |
Mito lysate | 54.3 | 508.4 | 9.4 | 1 | 100 |
Lysate S130 | 29.3 | 463.1 | 15.8 | 1.7 | 91 |
Pooled TEC | 1.7 | 885 | 520.6 | 55.4 | 174 |
One unit activity is defined as 1 µmol of UMP incorporated at 30°C in 30 min under standard assay conditions.