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. 2024 May 7;7(7):e202302384. doi: 10.26508/lsa.202302384

Figure 4. GDF15 modulates Adcy3 and Hdac6 expression.

Figure 4.

(A) Representative confocal micrographs of primary cilia at the apical side of whole mount preparations of the E18 GE of the given genotype upon immunostaining with the indicated antibodies. Note that the expression of ADCY3 but not acTub appeared to be modified according to genotype/treatment. (B) Quantification of ADCY3 fluorescence intensity in cilia of whole mount preparations of the GE and coronal sections of the HP of E18 mice. (C) Representative confocal micrographs of primary cilia at the apical side of whole mount preparations of the E18 GE of the given genotype treated as indicated for 24 h and processed for immunostaining with ARL13B (red) antibodies to identify primary cilia. DAPI nuclear counterstaining is visualized in blue. Scale bars indicate 10 μm. (D, E) Quantification of the effect of the treatments on cilia length (D) and thickness (E). Bars indicate mean ± SEM. Each data point represents the average of data collected from one individual animal. * indicate significance as indicated (*P < 0.05, **P < 0.01, ***P < 0.001); ns, not significant.