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. 2024 May 7;7(7):e202302384. doi: 10.26508/lsa.202302384

Figure S1. Characterization of primary cilia in the GE/V-SVZ.

Figure S1.

(A) Immunofluorescent micrographs of the lateral SVZ in coronal sections of adult WT mice, labelled for cilia marker ARL13B (green), and GFRAL (red). DAPI was used for nuclear counterstain. Scale bar = 20 μm. (B) Immunofluorescent micrographs of whole mounts of the E18 GE labelled for ARL13B and γ-tubulin show no difference in γ-tubulin localization in relation to the cilium. Scale bars = 10 μm. (C) Immunofluorescent micrographs of whole mounts of the E18 GE labelled for acTub and ADCY3 showing two emerging ependymal cells (white arrows), demonstrating a clear difference between primary and emerging motile cilia. DAPI was used for nuclear counterstain. Scale bar = 5 μm.