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(A) Gate for particles with cell size plotted in forward and side scatter channels. (B) Gate for living cells (propidium iodide negative, PI−) plotted in PI and FSC channels. Cells above the gate (PI+) are considered dead. (C, D) Gate for Prominin-1+ (P+) cells, using cells without (C) and with Prominin-1-APC antibody (D), plotted on PI and APC channels. All graphs show cells dissociated from a WT E18 GE.
