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. 2024 Feb 25;11(17):2309032. doi: 10.1002/advs.202309032

Figure 1.

Figure 1

Single‐cell RNA sequencing of healthy and diseased human AF. A) Visualization of clustering by UMAP plot of healthy and diseased AF samples (n = 13) using 0.5 resolution. B) Quantification of AF cluster contribution shown as percentage of total AF cell count of the integrated data set. C) Top markers for each AF cluster visualized in a heat map. D) Visualization of clustering by split UMAP plot of healthy (n = 3) versus diseased (n = 10) AF samples. E) Percentage of cells from each condition (healthy versus diseased) are shown in a pie chart. F) CellChat analysis was performed to interrogate cell‐cell communication patterns between clusters in AF. Overall outgoing and incoming signal strength of each cluster was visualized in a scatter plot. G) Relative strength of all enriched signals across AF clusters was visualized in a heat map. For all panels: GenC (general chondrocytes), RegC (regulatory chondrocytes), FC (fibrochondrocytes), HomC (homeostatic chondrocytes), HomFibro (homeostatic fibroblasts), PC (progenitor cells), SC (stem cells), BVC (blood vessel cells) and DAC (disease‐associated chondrocytes). Healthy = grade II; Diseased = grade II‐III, grade III and grade III‐IV.