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Human Primer Sequences Used for qPCR
| Genes | Forward Primer (5′−3′) | Reverse Primer (5′−3′) |
|---|---|---|
| SLIT1 | GCGGCTATAAGGGTCGAGACT | TGTTCACCCCACAGGTTGGT |
| SLIT2 | GGTGAACACTGCGACATCGA | GAGGACCATGGGTGGAGAAA |
| SLIT3 | GAGAGGGAGCTTTCGATGGA | GTCATTACTCACACAGCCGATCA |
| ROBO1 | TAGTTCTTCGGACGGCTCCTT | GTGGGCCTAGGGCACTGA |
| ROBO2 | TCCATGGCTTGCTGATTCTTG | CCATCTGAGAGCATCGTTGCT |
| ROBO3 | GCCAGTACGCTCCTCCAGAGT | CTAGGCAGCTCAGTTCACAAGAAG |
| ROBO4 | GCAGCTGAGGGCTACCTTGA | CTGTGATCCATCCTGTGTTTTAGG |
| β-ACTIN | GGGAAATCGTGCGTGACATT | GGAACCGCTCATTGCCAAT |
