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. Author manuscript; available in PMC: 2024 May 8.
Published in final edited form as: J Vis Exp. 2024 Jan 12;(203):10.3791/65589. doi: 10.3791/65589

Table 1: Standard and HeLa S3 peptide LC-TIMS-ToF MS/MS characteristics.

Target and observed peptide list, including experimental properties (i.e., retention time, m/z, 1/Ko, and LC peak areas).

Target Peptides Histone Standard Mix HeLa S3
Amino acid position Peptide/mod Charge State m/z Reporter Ions RT (min) m/z experimental 1/k0 LC peak area RT (min) m/z experimental 1/k0 LC peak area
H3_01_3_8 TKQTAR
unmod 1 816.4574 14.3 816.4552 1.314 92098 14.3 816.4555 1.338 14192
2 408.7323 659.38, 342.20 408.7326 0.746, .769 408.7325 .771, .792
K4me1 1 830.473
2 415.7402
K4me2 1 788.4625
2 394.7349
K4me3 1 802.4781
2 401.7427
K4ac 1 802.4417
2 401.7245
H3_02_9_17 KSTGGKAPR
unmod 1 1069.6 16.7 1069.5965 1.585, 1.607, 1.616 171405 16.5 5083
2 535.3036 829.45, 742.42, 641.37, 584.35, 527.33, 328.19, 429.23, 486.26, 543.28 535.3028 0.919 535.3033 0.921
3 357.2049
K9me1 1 1083.6157 17.9 3056
2 542.3115 542.3113 0.91
3 361.8767
K9me2 1 1041.6051 13.2 8285
2 521.3062 829.45, 742.42, 641.37, 584.35, 527.33, 300.19, 401.24, 458.26, 515.28 521.3065 0.847
3 347.8732
K9me3 1 1055.6207 13.1 3468
2 528.314 528.3144 .856, .876
3 352.5451
K9ac 1 1055.5844
2 528.2958 829.45, 742.42, 641.37, 584.35, 527.33, 314.17, 415.22, 472.24, 529.26
3 352.533
K14ac 1 1055.5844
2 528.2958 815.44, 728.41, 627.36, 570.34, 513.31, 328.19, 429.23, 486.26, 543.28
3 352.533
K9me1K14ac 1 1069.6
2 535.3036 815.44, 728.41, 627.36, 570.34, 513.31, 342.21, 443.25, 500.28, 557.30
3 357.2049
K9me2K14ac 1 1027.5894
2 514.2984
3 343.2013
K9me3K14ac 1 1041.6051
2 521.3062 815.44, 728.41, 627.36, 570.345, 513.31, 314.21, 415.26, 472.28, 529.30
3 347.8732
K9acK14ac 1 1041.5687
2 521.288
3 347.8611
H3_03_18_26 KQLATKAAR
unmod 1 1154.6892 21.1 1154.6895 1.728 1749137 21 1154.6826 1.725 737276
2 577.8482 914.54, 786.48, 673.40, 602.36, 501.31, 369.21, 482.30, 553.33, 654.38 577.8482 .918, .966 577.8478 .919, .967
3 385.5679
K23me1 1 1168.7048
2 584.856
3 390.2398
K18me1 1 1168.7048
2 584.856
3 390.2398
K18me1K23me1 1 1182.7205
2 591.8639
3 394.9117
K18ac 1 1140.6735
2 570.8404 914.54, 786.48, 673.40, 602.36, 501.31, 355.20, 468.28, 539.32, 640.37
3 380.896
K23ac 1 1140.6735 20.3 1140.672 1.709 204353
2 570.8404 900.53, 772.47, 659.38, 588.35, 487.30, 369.21, 482.30, 553.33, 654.38 570.8405 .910, .957
3 380.896
K18acK23ac 1 1126.6579
2 563.8326
3 376.2241
H3_04_27_40 KSAPATGGVKKPHR
unmod 2 829.4728 17.7 829.4698 1.118 247198
3 553.3176 1417.79, 1330.76, 1259.72, 1162.67, 1091.63, 990.58, 933.56, 876.54, 777.47, 593.35, 328.19, 399.22, 496.28, 567.31, 668.36, 725.38, 782.40, 881.47 553.3176 0.891
4 415.2401
K36me1 2 836.4807
3 557.9895 1431.81, 1344.78, 1273.74, 1176.69, 1105.65, 1004.60, 947.58, 890.56, 791.49, 328.19, 399.22, 496.28, 567.31, 668.36, 725.38, 782.40, 881.47
4 418.744
K27me1 2 836.4807 18.5 836.478 1.123, 1.141 7173
3 557.9895 1417.79, 1330.76, 1259.72, 1162.67, 1091.63, 990.58, 933.56, 876.54, 777.47, 342.21, 413.24, 510.30, 581.33, 682.38, 739.40, 796.42, 895.49 557.9894 .890, .900, .909
4 418.744
K27me2 2 815.4754
3 543.986 1417.79, 1330.76, 1259.72, 1162.67, 1091.63, 990.58, 933.56, 876.54, 777.47, 300.19, 371.23, 468.28, 539.32, 640.37, 697.39, 754.41, 853.48
4 408.2413
K36me2 2 815.4754
3 543.986 1389.80, 1302.76, 1231.73, 1134.67, 962.59, 905.57, 848.55, 749.48, 328.19, 399.22, 496.28, 496.28, 567.31, 668.36, 725.38, 782.40, 881.47
4 408.2413
K27me3 2 822.4832 15.7 4310
3 548.6579 1417.79, 1330.76, 1259.72, 1162.67, 1091.63, 990.58, 933.56, 876.54, 777.47, 314.21, 385.24, 482.30, 553.33, 654.38, 711.40, 768.43, 867.49 548.6568 0.896
4 411.7452
K36me3 2 822.4832
3 548.6579 1403.81, 1316.78, 1245.74, 1148.69, 1077.65, 976.61, 919.56, 862.56, 763.35, 328.19, 399.22, 496.28, 567.31, 668.36, 715.38, 782.40, 881.47
4 411.7452
K27me2K36me1 2 822.4832
3 548.6579 1431.81, 1344.78, 1273.74, 1179.69, 1105.65, 1004.60, 947.58, 890.56, 791.49, 300.19, 371.23, 468.28, 539.32, 640.37, 697.39, 754.41, 853.62
4 411.7452
K27me1K36me2 2 822.4832
3 548.6579 1389.80, 1302.76, 1231.73, 1134.67, 1063.64, 962.59, 905.55, 848.55, 749.48, 342.21, 413.24, 510.30, 581.33, 682.38, 739.40, 895.49
4 411.7452
K27me1K36me1 2 843.4885
3 562.6614
4 422.2479
K27me3K36me1 2 829.491
3 553.3298 1431.81, 1344.78, 1273.74, 1176.69, 1105.65, 1004.60, 947.58, 890.56, 791.49, 314.21, 385.24, 482.30, 553.33, 654.38, 711.40, 768.43, 867.49
4 415.2491
K27me1K36me3 2 829.491
3 553.3298 1409.81, 1316.78, 1245.74, 1148.69, 1077.65, 976.61, 976.61, 919.58, 862.56, 763.49, 342.21, 413.24, 510.30, 581.33, 682.38, 739.40, 796.42, 896.49
4 415.2491
K27me2K36me2 2 801.4779
3 534.6544
4 401.2426
K27me3K36me2 2 808.4857
3 539.3263
4 404.7465
K27me2K36me3 2 808.4857
3 539.3263
4 404.7465
K27me3K36me3 2 815.4936
3 543.9981
4 408.2504
K27ac 2 822.465
3 548.6458
4 411.7361
H3_04v3_27_40 KSAPSTGGVKKPHR
unmod 2 837.4703
3 558.6493
4 419.2388
K36me1 2 844.4781
3 563.3212
4 422.7427
K27me1 2 844.4781
3 563.3212
4 422.7427
K27me2 2 823.4728
3 549.3176 1433.79, 1346.75, 1275.72, 1178.66, 1091.63, 990.58, 933.56, 876.54, 777.47, 593.35, 300.19, 371.23, 468.28, 555.28, 656.36, 713.38, 770.40, 869.47, 1053.59
4 412.2401
K36me2 2 823.4728
3 549.3176 1405.79, 1318.76, 1247.72, 1150.67, 1063.64, 962.59, 905.57, 848.55, 749.48, 565.36, 328.19, 399.22, 496.28, 583.31, 684.36, 741.38, 798.40, 897.47, 1081.59
4 412.2401
K27me3 2 830.4807
3 553.9895
4 415.744
K36me3 2 830.4807
3 553.9895
4 415.744
K27me2K36me1 2 830.4807
3 553.9895
4 415.744
K27me1K36me2 2 830.4807
3 553.9895
4 415.744
K27me1K36me1 2 851.4859
3 567.9931
4 426.2466
K27me3K36me1 2 837.4885
3 558.6614
4 419.2479
K27me1K36me3 2 837.4885
3 558.6614
4 419.2479
K27me2K36me2 2 809.4754
3 539.986
4 405.2413
K27me3K36me2 2 816.4832
3 544.6579
4 408.7452
K27me2K36me3 2 816.4832
3 544.6579
4 408.7452
K27me3K36me3 2 823.491
3 549.3298
4 412.2491
K27ac 2 830.4625
3 553.9774
4 415.7349
H3_05_41_49 YRPGTVALR
unmod 1 1088.6211 18.9 2910608 18.8 232847
2 544.8142 544.8136 0.868 544.8148 0.87
3 363.5452
Y41ph 1 1168.5874
2 584.7973
3 390.2007
H3_06_54_63 YQKSTELLIR
unmod 1 1362.7627 25.5 93690 25.4 63192
2 681.885 681.884 1.012 681.8846 1.013
3 454.9258
4 341.4461
K56me1 1 1376.7784
2 688.8928
3 459.5976
4 344.9501
K56me2 1 1334.7678
2 667.8875
3 445.5941
4 334.4474
K56me3 1 1348.7835
2 674.8954
3 450.266
4 337.9513
K56ac 1 1348.7471
2 674.8772
3 450.2539
4 337.9422
H3_07_73_83 EIAQDFKTDLR
unmod 1 1447.7427 29.5 65430 29.3 119424
2 724.375 724.3749 1.022 724.3744 1.022
3 483.2524
4 362.6911
K79me1 1 1461.7584
2 731.3828
3 487.9243
4 366.195
K79me2 1 1419.7478
2 710.3775
3 473.9208
4 355.6924
K79me3 1 1433.7634
2 717.3854
3 478.5927
4 359.1963
K79ac 1 1433.7271
2 717.3672
3 478.5805
4 359.1872
H3_08_117_128 VTIMPKDIQLAR
unmod 1 1496.8505 30.5 100245 30.3 55421
2 748.9289 748.9275 1.021, 1.077 748.9276 1.021, 1.077
3 499.6217
4 374.9681
K122ac 1 1482.8348
2 741.9211
3 494.9498
4 371.4642
H3_09u_64_135 unmod
KLPFQR 1 900.5301 23.7 900.528 1.406, 1.428 1024986 23.6 900.5261 1.404, 1.429 239181
2 450.7687 660.38, 547.30, 450.25, 354.24, 451.29, 598.36 450.7693 0.808 450.7688 0.807
3 300.8482
RIRGER 1 842.4955 16.2 2722
2 421.7514 421.7505 .779, .794
3 281.5033
IRGERA 1 757.4315
2 379.2194
3 253.1487
H4_01_4_17 GKGGKGLGKGGAKR
unmod 1 1550.9013 19.6 419558 19.5 250690
2 775.9543 775.9539 1.122, 1.140 775.9536 1.121, 1.140
3 517.6386 517.6376 .799, .829 517.6387 .815, .832
4 388.4808
K5ac 1 1536.8856
2 768.9464 1253.73, 1196.71, 1139.69, 284.16, 341.18, 398.20
3 512.9667
4 384.9769
K8ac 1 1536.8856
2 768.9464 955.57, 898.46, 785.46, 728.44, 298.17, 355.20, 412.22, 582.32, 639.35, 752.43, 809.45
3 512.9667
4 384.9769
K12ac 1 1536.8856
2 768.9464 941.55, 884.53, 771.45, 714.43, 544.32, 487.30, 430.28, 359.24, 596.34.653.36, 766.45, 823.47
3 512.9667
4 384.9769
K16ac 1 1536.8856 19 32497
2 768.9464 530.30, 473.28, 416.26, 345.22, 1007.59, 1121.63, 1192.67 768.9464 1.112, 1.128
3 512.9667 512.9662 .805, .824, .837
4 384.9769
K5acK8ac 1 1522.87
2 761.9386 1239.72, 1182.70, 1125.67, 955.57, 989.55, 785.46, 728.44, 544.32, 487.30, 359.24, 284.16, 341.18, 398.20, 568.31, 625.33, 738.41, 795.44, 979.56, 1036.58, 1093.6, 1164.64
3 508.2948
4 381.4729
K5acK12ac 1 1522.87
2 761.9386 1239.72, 1182.70, 1125.67, 941.55, 884.53, 771.45, 714.43, 544.32, 487.30, 430.28, 359.24, 284.16, 341.18, 398.20, 582.32, 639.35, 752.43, 809.45, 979.56, 1036.48, 1093.6, 1164.64
3 508.2948
4 381.4729
K5acK16ac 1 1522.87
2 761.9386 1239.72, 1182.70, 1125.67, 941.55, 884.53, 771.45, 714.43, 530.30, 473.28, 416.26, 345.22, 284.16, 341.18, 398.20, 582.32, 639.35, 752.43, 809.45, 993.57, 1050.59, 1107.62, 1178.65
3 508.2948
4 381.4729
K8acK12ac 1 1522.87
2 761.9386 941.55, 884.53, 771.45, 714.43, 544.32, 487.30, 430.28, 359.24, 298.18, 355.20, 412.22, 582.32, 639.35, 752.43, 809.45, 979.56, 1036.58, 1093.6, 1164.64
3 508.2948
4 381.4729
K8acK16ac 1 1522.87
2 761.9386 941.55, 884.53, 771.45, 714.43, 530.30, 473.28, 416.26, 345.22, 298.18, 355.20, 412.22, 582.32, 639.35, 752.43, 809.45, 993.57, 1050.59, 1107.62, 1178.65
3 508.2948
4 381.4729
K12acK16ac 1 1522.87
2 761.9386 927.54, 870.52, 757.43, 700.41, 530.30, 473.28, 416.26, 345.22, 298.18, 355.20, 412.22, 596.34, 653.36, 766.45, 823.47, 993.57, 1050.59, 1107.62, 1178.65
3 508.2948
4 381.4729
K5acK8acK12ac 1 1508.8543
2 754.9308 941.55, 884.53, 771.45, 714.43, 544.32, 487.30, 430.28, 359.24, 341.18, 568.31, 625.33, 738.41, 965.54, 1022.56, 1079.58, 1150.62
3 503.623
4 377.969
K5acK8acK16ac 1 1508.8543
2 754.9308 941.55, 884.53, 771.44, 714.43, 568.31, 625.33, 738.41, 795.44
3 503.623
4 377.969
K5acK12acK16ac 1 1508.8543
2 754.9308 927.54, 870.52, 757.43, 700.41, 582.32, 639.35, 752.43, 809.45
3 503.623
4 377.969
K8acK12acK16ac 1 1508.8543
2 754.9308 1211.69, 1154.66, 1097.64, 927.54, 870.52, 757.43, 700.41, 298.18, 355.20, 412.22, 582.32, 639.35, 752.43, 809.45
3 503.623
4 377.969
K5acK8acK12acK16ac 1 1494.8387
2 747.923
3 498.9511
4 374.4651
H4_02_20_23 KVLR
unmod 1 627.4188 20.8 627.4178 1.215 835125 20.8 627.4204 1.213 2079
2 314.213 314.2138 .720, .735
K20me1 1 641.4345 22.3 641.4339 1.23 35976
2 321.2209
K20me2 1 599.4239 16.5 599.4245 1.172 283059
2 300.2156 300.2157 0.727
K20me3 1 613.4395 16.5 3838
2 307.2234 307.223 .692, .707, .723
K20ac 1 613.4032
2 307.2052
H4_03_24_35 DNIQGITKPAIR
unmod 1 1437.806 21.3 548144 21.2 337235
2 719.4066 719.4054 .992, 1.013, 1.026 719.4055 .994, 1.018, 1.031
3 479.9402 479.9387 0.818 479.9401 .778, .797, .816
4 360.207
H4_04_40_45 RGGVKR
unmod 1 784.4788 14 83319 13.7 12188
2 392.743 392.7429 0.758 392.7437 0.749
K44ac 1 770.4631
2 385.7352
H4_05_79_92 KTVTAMDVVYALKR
unmod 1 1762.9771 43.9 27760
2 881.9922 881.9916 1.234
3 588.3306
4 441.4997
H4_06u_46_102 unmod
ISGLIYEETR 1 1236.647 30.4 1236.648 1.723 630923 30.2 1236.6429 1.715 101001
2 618.8272 618.8266 0.951 618.826 0.952
3 412.8872
4 309.9172
GVLKVFLENVIR 1 1498.8991
2 749.9532
3 500.3046
4 375.4802
DAVTYTEHAKR 1 1402.6961 18.4 39543 18.3 1119
2 701.8517 701.8503 1.008 701.8525 1.01
3 468.2369 468.2373 0.758
4 351.4295
TLYGFGG 1 770.3719 27.4 770.3708 1.283, 1.348 276638 27.1 770.3721 1.284, 1.303 17029
2 385.6896
3 257.4622
4 193.3484
HH2A_01m1_36_42 KGNYAER
unmod 1 949.4738 17.1 949.4704 1.453 543640 17 949.4721 1.45 7694
2 475.2405 475.2401 .812, .841 475.241 0.84
K36ac 1 935.4581
2 468.2327
HH2A_01m3_36_42 KGNYSER
unmod 1 965.4687
2 483.238
K36ac 1 951.453
2 476.2302
HH2A_01oX_36_42 KGHYAER
unmod 1 972.4897
2 486.7485
K36ac 1 958.4741
2 479.7407
HH2A_02m1_4_11 GKQGGKAR
unmod 1 969.5476 15.5 969.5441 1.486 565276 15.4 969.5492 1.475, 1.486 51680
2 485.2774 672.38, 544.32, 487.30, 430.28, 298.18, 426.23, 483.26, 540.28 485.2762 0.875 485.2779 0.873
K5ac 1 955.5319
2 478.2696 284.16, 412.22, 469.24, 526.26, 672.28, 544.32, 487.30, 430.28
K9ac 1 955.5319
2 478.2696 658.36, 530.30, 473.28, 416.26, 298.18, 426.23, 483.26, 540.28
K5acK9ac 1 941.5163
2 471.2618
K9me1 1 983.5632
2 492.2853 686.40, 558.34, 501.32, 444.30, 298.18, 426.23, 483.26, 543.28
K5me1 1 983.5632
2 492.2853 672.38, 544.32, 487.30, 430.28, 312.20, 440.25, 497.28, 554.30
HH2A_02oJ_4_11 GKQGGKVR
unmod 1 997.5789
2 499.2931
K5ac 1 983.5632
2 492.2853 700.41, 572.35, 515.33, 458.31, 284.16, 412.22, 469.24, 526.26
K9ac 1 983.5632
2 492.2853 686.39, 558.34, 501.31, 444.29, 298.18, 426.23, 483.26, 543.28
K5acK9ac 1 969.5476
2 485.2774 686.39, 558.34, 501.31, 444.29, 284.16, 412.22, 469.24, 526.26
K9me1 1 1011.5945
2 506.3009 714.43, 586.37, 529.35, 472.33, 298.18, 426.23, 483.26, 543.28
K5me1 1 1011.5945
2 506.3009 700.41, 572.35, 515.33, 458.31, 312.20, 440.25, 497.28, 554.30
HH2A_02oX_4_11 GKTGGKAR
unmod 1 942.5367
2 471.772
K5ac 1 928.521
2 464.7642 645.37, 544.32, 487.30, 430.28, 284.16, 385.21, 442.23, 499.25
K9ac 1 928.521
2 464.7642 631.35, 530.30, 473.28, 416.26, 298.18, 399.22, 456.25, 513.27
K5acK9ac 1 914.5054
2 457.7563
K9me1 1 956.5523 16.6 11778
2 478.7798 659.39, 558.34, 501.32, 444.30, 298.18, 399.22, 456.25, 513.27 478.779 .843, .859
K5me1 1 956.5523
2 478.7798 645.37, 544.32, 487.30, 430.28, 312.20, 413.24, 470.27, 527.29
HH2A_03m1_1_11 SGRGKQGGKAR
unmod 1 1269.7022 14 514
2 635.3547 635.3556 0.95
3 423.9056
S1ac 1 1311.7127
2 656.36
3 437.9091
K5ac 1 1255.6865
2 628.3469
3 419.2337
HH2A_03oV_1_19 AGGKAGKDSGKAKAKAVSR
unmod 2 1062.0946
3 708.3988
4 531.5509
5 425.4422
K4ac 2 1055.0867
3 703.7269
4 528.047
5 422.6391
K7ac 2 1055.0867
3 703.7269
4 528.047
5 422.6391
K11ac 2 1055.0867
3 703.7269
4 528.047
5 422.6391
K15ac 2 1055.0867
3 703.7269
4 528.047
5 422.6391
K4acK7ac 2 1048.0789
3 699.055
4 524.5431
5 419.8359
K4acK11ac 2 1048.0789
3 699.055
4 524.5431
5 419.8359
K4acK15ac 2 1048.0789
3 699.055
4 524.5431
5 419.8359
K7acK11ac 2 1048.0789
3 699.055
4 524.5431
5 419.8359
K7acK15ac 2 1048.0789
3 699.055
4 524.5431
5 419.8359
K11acK15ac 2 1048.0789
3 699.055
4 524.5431
5 419.8359
K7acK11acK15ac 2 1041.0711
3 694.3831
4 521.0392
5 417.0328
K4acK11acK15ac 2 1041.0711
3 694.3831
4 521.0392
5 417.0328
K4acK7acK15ac 2 1041.0711
3 694.3831
4 521.0392
5 417.0328
K4acK7acK11ac 2 1041.0711
3 694.3831
4 521.0392
5 417.0328
K4acK7acK11acK15ac 2 1034.0633
3 689.7113
4 517.5353
5 414.2297
HH2A_03oZ_1_19 AGGKAGKDSGKAKTKAVSR
unmod 2 1077.0998
3 718.4023
4 539.0536
5 431.4443
K4ac 2 1070.092
3 713.7304
4 535.5496
5 428.6412
K7ac 2 1070.092
3 713.7304
4 535.5496
5 428.6412
K11ac 2 1070.092
3 713.7304
4 535.5496
5 428.6412
K15ac 2 1070.092
3 713.7304
4 535.5496
5 428.6412
K4acK7ac 2 1063.0842
3 709.0586
4 532.0457
5 425.838
K4acK11ac 2 1063.0842
3 709.0586
4 532.0457
5 425.838
K4acK15ac 2 1063.0842
3 709.0586
4 532.0457
5 425.838
K7acK11ac 2 1063.0842
3 709.0586
4 532.0457
5 425.838
K7acK15ac 2 1063.0842
3 709.0586
4 532.0457
5 425.838
K11acK15ac 2 1063.0842
3 709.0586
4 532.0457
5 425.838
K7acK11acK15ac 2 1056.0764
3 704.3867
4 528.5418
5 423.0349
K4acK11acK15ac 2 1056.0764
3 704.3867
4 528.5418
5 423.0349
K4acK7acK15ac 2 1056.0764
3 704.3867
4 528.5418
5 423.0349
K4acK7acK11ac 2 1056.0764
3 704.3867
4 528.5418
5 423.0349
K4acK7acK11acK15ac 2 1049.0685
3 699.7148
4 525.0379
5 420.2318
HH2A_04m1_12_17 AKAKTR
unmod 1 842.5094 17 842.5092 1.428 60315
2 421.7583 531.33, 460.29, 312.20, 383.23, 276.17 421.7597 .772, .793
K13ac 1 828.4938
2 414.7505
K15ac 1 828.4938
2 414.7505
K13acK15ac 1 814.4781
2 407.7427
K15me1 1 856.5251
2 428.7662 531.33, 460.29, 326.21, 397.25
K13me1 1 856.5251
2 428.7662 545.34, 474.31, 312.20, 383.23
HH2A_04m3_12_17 AKAKSR
unmod 1 828.4938 16.9 828.49 1.411 1140381 16.8 828.4922 1.409 44577
2 414.7505 414.7502 0.788 414.7502 0.787
K13ac 1 814.4781
2 407.7427 517.31, 446.27, 298.18, 369.21
K15ac 1 814.4781
2 407.7427 503.29, 432.26, 312.20, 383.23
K13acK15ac 1 800.4625
2 400.7349
K15me1 1 842.5094
2 421.7583 531.33, 460.29, 312.20, 383.23, 262.15
K13me1 1 842.5094
2 421.7583 517.31, 446.27, 326.21, 397.25
HH2A_05m1_72_77 DNKKTR
unmod 1 929.5051 16.2 929.5022 1.448 162881 16.1 929.5048 1.433, 1.446 18624
2 465.2562 465.2559 0.816 465.2567 .805, .817
K74ac 1 915.4894
2 458.2483
HH2A_06_82_88 HLQLAIR
unmod 1 906.5519 20.9 16685936 20.8 725428
2 453.7796 453.7796 0.823 453.7799 0.825
3 302.8555
HH2A_07v_1_88 unmod
H2AZ.AGGKAGKDSGKAKTKAVSR 2 1077.0998
3 718.4023
H2AY.SAKAGVIFPVGR 1 1313.7576
2 657.3824
3 438.5907
H2AX.GKTGGKAR 1 942.5367
2 471.772
3 314.8504
HH2A_08u_4_99 unmod
HLQLAVR 1 892.5363 19.8 19140
2 446.7718 446.7721 0.815
3 298.1836
GGKKKSTKTSR 1 1457.8322
2 729.4197
3 486.6156
SGKKKMSKLSR 1 1529.872
2 765.4396
3 510.6288
IHRHLKTR 1 1172.7011
2 586.8542
3 391.5719
IHRHLKSR 1 1158.6854
2 579.8463
3 386.9
NDEELNKLLGR 1 1412.738 27.5 12177
2 706.8726 706.8716 0.996
3 471.5842
AGLQFPVGR 1 1000.5574 23.4 1000.5555 1.444, 1.478, 1.510 14000051 23.2 1000.5538 1.475, 1.506 737499
2 500.7823 500.7814 .836, .855 500.7828 .837, .855
3 334.1907
VHR 1 467.2725 11.1 467.2726 1.009 68980
2 234.1362
3 156.4242
LLR 1 457.2832
2 229.1416
3 153.0944
IHPELLAKKR 1 1372.8311
2 686.9192
3 458.2819
YIKKGHPKYR 1 1513.8525
2 757.4299
3 505.289
HH2B_01v_1_29 unmod
1C/1K.PEPAKSAPAPKKGSKKAVTKAQKKDGKKR 3 1226.0397
4 919.7816
1H.PDPAKSAPAPKKGSKKAVTKAQKKDGKKR 3 1221.3679
4 916.2777
2F.PDPAKSAPAPKKGSKKAVTKVQKKDGKKR 3 1230.7116
4 923.2855
1B.PEPSKSAPAPKKGSKKAITKAQKKDGKKR 3 1236.0433
4 927.2843
1N.PEPSKSAPAPKKGSKKAVTKAQKKDGKKR 3 1231.3714
4 923.7804
1D.PEPTKSAPAPKKGSKKAVTKAQKKDGKKR 3 1236.0433
4 927.2843
1M.PEPVKSAPVPKKGSKKAINKAQKKDGKKR 3 1253.7309
4 940.55
1L.PELAKSAPAPKKGSKKAVTKAQKKDGKKR 3 1231.3835
4 923.7895
HH2B_02u_1_100 unmod
LAHYNKR
unmod 1 1013.5527 19 815519 18.9 103031
2 507.28 507.2788 0.883 507.2803 0.883
3 338.5224
LPHYNKR 1 1039.5683
2 520.2878
3 347.1943
EIQTAVR 1 872.4836 18.6 872.4829 1.386 754301
2 436.7454 436.7443 0.797
3 291.4994
IAGEASR 1 759.3995 17.5 759.3988 1.3 214007 17.4 759.3988 1.302 5806
2 380.2034 380.2035 0.746
3 253.8047
IASEASR 1 789.4101
2 395.2087
3 263.8082
LRTEVPRLPR 1 1292.7797
2 646.8935
3 431.5981