Fig. 1. Chemical structure of parent compounds and activity-based probes with their determined IC50 and Ki accordingly. Fluorescent Polarisation assay were performed for IC50 (30 minutes incubation against USP30), kobs/I, and kinact/Ki determination of IMP-2587, and IMP-2586 against USP30 using Ub-Lys(TAMRA)-Gly (Fig. S1, ESI†).
