Fig. 4. (a) Workflow of HA-tagged USP30 WT or C77S transfection system and IMP-2587/IMP-2586 labelling in HEK293T cells. HEK293T cells were transfected to overexpress HA-tagged USP 30 WT or catalytic CS mutants, and pulldown following treatment with IMP-2587 (b) or IMP-2586 (c) for 10 min. (PD: Pull-Down, TL: Total Lysate).