Table 3.
Instrument-Free Technologies With the Potential to be Used as HCV Assays a
| Visby Medicalb | Sherlock Biosciencesc | Ustar PortNATd | Midge Medical Minooe | |
|---|---|---|---|---|
| Sample preparation | Thermal and chemical lysis | Thermal and chemical lysis | Thermal and chemical lysis | Thermal and chemical lysis |
| Amplification method | RT-PCR | Isothermal/CRISPR | Proprietary isothermal amplification technology | RPA |
| Turnaround time, min | 30 | 15 | 25 | NA |
| Tests menu, commercially available | SARS-CoV-2, Flu A/B; STDs | NA | SARS-CoV-2 | NA |
| Power supply | Needs electricity | Battery powered | Battery powered | Battery powered |
| Tests in development | NA | STDs, respiratory diseases | HIV, CT/NG, Flu | SARS-CoV-2, HIV, M. tuberculosis, Flu, herpes virus, Ebola virus |
Abbreviations: CRISPR, clustered, regularly interspaced, short palindromic repeat; CT/NG, Chlamydia trachomatis, Neisseria gonorrhea, Trichomonas vaginalis; Flu, influenza; HCV, hepatitis C virus; HIV, human immunodeficiency virus; M. tuberculosis, Mycobacterium tuberculosis; RPA, recombinant polymerase amplification; RT-PCR, reverse transcription polymerase chain reaction; SARS-CoV-2, severe acute respiratory syndrome coronavirus 2; STDs, sexually transmitted diseases.
aThe technologies selected are illustrative examples to demonstrate different types of testing systems. The information about test pipeline is taken from company websites.