Extended Data Figure 5 |. Intraperitoneally-injected LIPSTIC substrate reaches cells in multiple tissues.

(A) Steady state Rosa26^uLIPSTIC/WT.Clec9a^CreER/WT or Rosa26^uLIPSTIC/WT.Clec9a^WT/WT control mice were injected i.p. with the LIPSTIC substrate and its loading onto DCs was achieved in all analyzed tissues. (B-C) I.p. injection of the LIPSTIC substrate reaches the brain in Rosa26^uLIPSTIC/WT.Cx3xr1^CreERT2/WT mice. (B) Flow cytometry gating strategy to analyze CX3CR1-expressing microglia in the brain. To discriminate resident from circulating immune cells in the brain, α-CD45 antibody was injected intravenously to mark the latter. (C) SrtA expression was detected in ~80% of CD11b⁺CX3CR1⁺ cells, 68% of which acquired i.p.-administered LIPSTIC substrate.