Extended Data Fig. 7. WRN inhibition induces apoptosis, a p53 response and cell cycle arrest.
a. Xenograft growth of SW48 cells treated p.o. twice daily with compound 3 at 15, 50 or 150 mg/kg. Mice were randomized (n = 7). b. Mean total blood concentration of compound 3 performed on the last day of the efficacy experiment. Data are average of n = 3 mice per time point ± SEM c. Immunoblot for WRN, pATM (S1981), pCHK2 (T68), pATR (Y1989), p21, pH2AX (S139) and actin 3 h post-last treatment with compound 3 on the last day of the efficacy experiment (i.e., day 13 post start of treatment).