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. 2024 Apr 10;629(8011):474–480. doi: 10.1038/s41586-024-07295-2

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© The Author(s) 2024

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Extended Data Fig. 12 — a, Phosphorylation of µOR∆7-182 C/276 C by GRK5 for DEER spectroscopy. Anion exchange chromatography (MonoQ) was used to find the best condition for GRK5 phosphorylation of the µOR. b, smFRET distributions of GRK5-phosphorylated µOR∆7-182 C/273C-Cy3/Cy5 (µORp-Cy3/Cy5) in the presence of 20 µM of C8-PIP2. The grey shaded areas are the DAMGO alone condition for comparison.

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