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. 2024 Apr 10;629(8011):474–480. doi: 10.1038/s41586-024-07295-2

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© The Author(s) 2024

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Extended Data Fig. 2 — a, Ligand efficacy of µOR∆7 is determined by BRET-based assays. Error bars represent s.d. from n = 6 samples. b, Ligand-binding affinity of µOR∆7 is determined by radioligand binding assay. The cell membrane of sf9 cells expressing µOR-WT or µOR∆7 was extracted and used for competition binding. The hot ligand is ³H-naloxone. Error bars represent s.d. from n = 4 samples.

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