Fig. 1.

A Protein levels of CST2 in the normal gastric mucosal epithelial cell line GES-1 and various human GC cell lines. B The expression levels of CST2 protein in GC tissues compared to adjacent normal gastric tissues. C The overexpression and knockdown efficiency of CST2 were confirmed using Western blot analysis. D The proliferation curves were employed to compare proliferation rates between CST2 overexpression cells and control cells. E EdU staining was employed to assess the effect of CST2 overexpression and knockdown on GC cell proliferation. F Wound healing in cell monolayers was assessed at 48 h, and wound closure rates were calculated. (G) Cell migration and invasion abilities were evaluated using Transwell assays. Data are displayed as the mean ± SD (n = 3). *P < 0.05, **P < 0.01, ***P < 0.001