Fig. 5. The septin family proteins exhibit elevated ubiquitination levels in an OspG-dependent manner.

a Ubiquitination of septin-family members in 293T cells co-expressing GFP, GFP-OspG or GFP-OspG K53A. Lysates were immunoprecipitated with Flag antibody and immunoblotted as indicated. Images are representative of n  =  5 independent experiments. b Ubiquitination of SEPT9 in Flag-SEPT9-expressing cells co-transfected with GFP-OspG and indicated HA-tagged ubiquitin constructs. Images are representative of n  =  2 independent experiments. The GFP vector was used as a control. K0 means ubiquitin in which all lysines were mutated to arginines and K6 means ubiquitin in which all lysines except Lys6 were mutated to arginines. K6R means ubiquitin in which only Lys6 were mutated to arginine. c Ubiquitination of SEPT9 in Flag-SEPT9-expressing cells co-transfected with GFP-OspG and indicated mutants. Images are representative of n  =  3 independent experiments. d 293T cells transfected with individual septins (SEPT2, SEPT6, and SEPT7) either alone or with GFP-OspG. Lysates were immunoprecipitated with Flag antibody and further immunoblotted as indicated. Images are representative of n  =  2 independent experiments.