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MeCP2 FRAP analysis in mutant cell lines
| Sample | Mobile fraction (plateau) | Immobile fraction | T50 (50% recovery) |
|---|---|---|---|
| WT J1 ESCs (n = 18 cells) | 75.2% ± 4.7% | 24.8% ± 4.7% | 75.5 s ± 13 s |
| DNMT TKO ESCs (n = 29 cells) | 97.5% ± 1.6% | 2.5% ± 1.6% | 9.9 s ± 0.3 s |
| WT Eset25 fibroblasts (n = 28 cells) | 74.8% ± 1.3% | 25.2% ± 1.3% | 105 s ± 28 s |
| 5KO fibroblasts (n = 26 cells) | 77.5% ± 5.6% | 22.5% ± 5.6% | 84.2 s ± 30 s |
