Figure 4.
Repair of UV-induced CPDs along the transcription initiation sites and along the upstream promoter of the human CDC2 gene. Data are for the upper (non-transcribed) strand and were analyzed with primer set Hbis. (A) Human foreskin fibroblasts were brought to quiescence by serum withdrawal, UV irradiated at 10 J/m2 and then incubated for the times indicated to allow repair. Lanes G+A, C+T and C (and lane G in panel B) are Maxam–Gilbert sequencing reactions. Transcription factor binding sites are shown on the right. The arrows indicate the transcription start sites and the direction of transcription. (B) Data for the non-transcribed strand in S phase-synchronized fibroblasts.