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. 2024 Apr 25;15:1388769. doi: 10.3389/fimmu.2024.1388769

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Copyright © 2024 Schuster, Braun, Chiang, Ludwig, Meng, Grätz, Kirchner, Proescholdt, Hau, Steinlein, Pfaffl, Riemenschneider and Reithmair

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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SmallRNA-Seq of 2D and 3D GBM models. (A) Graphical representation of the distribution of RNA reads from SmallRNA-Seq aligning to different EVRNAs from 2D or 3D culture and after IA and PP isolation; (B) PCA of total analyzed samples (n=48). (C) UpSet Plot shows the pairwise comparison of 2D and 3D with the number of unique and common differentially regulated miRNAs in six GBM cell lines. (D) Volcano plots of differentially expressed EVmiRNAs after IA and (E) PP isolation. (F) DGE analysis: Table lists the numbers of differentially expressed miRNAs after IA and PP isolation (Filter criteria: Base mean ≥ 100; log2FC ≥ 1 or log2FC ≤ -1; padj ≤ 0.1).