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. 2000 Oct 15;28(20):4037–4043. doi: 10.1093/nar/28.20.4037

Figure 2.

Figure 2

Effect of temperature and MgCl2 concentration on the self-cleavage activity of in vitro transcribed Pst3 and For6-2 isolates. (A) Pst3 RNA was incubated for 30 min at 35, 40 or 50°C with MgCl2 at concentrations from 0 to 40 mM. Independently of the temperature, the highest self-cleavage rates were observed with 5 mM MgCl2. (B) For6-2 RNA was incubated under similar conditions as the Pst3 RNA. The self-cleavage activity was highest in the range of 30–35°C with 5 mM MgCl2. The intact RNA (S) and the products of self-cleavage, 5′ (P1) and 3′ fragments (P2) are indicated.