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. 2024 May 9;5:0095. doi: 10.34133/cbsystems.0095

Fig. 7.

Fig. 7.

SH-SY5Y cell culture experiments in the microchannels of NCMCs with different widths. (A) The NCMCs with microchannel width are 3, 4, 5, 6, and 9 respectively. (B) The PDMS block broke into the gap between the soma chamber and the axon chamber. (C) The success rate of NCMC fabrication with different microchannel widths. (D) Fluorescence images of cells in the NCMC with different microchannel widths. Red dotted circles encircle leaking cells. Red scissors indicate axons passing through the microfluidic channel. (E) Images of dead cells in the NCMC with different microchannel widths. (F) Cell activity in the NCMCs with different widths. (G) Penetrated ratio: the proportion of microchannels penetrated by axons relative to the total number of microchannels. (H) Leakage ratio: the proportion of cells entering the axon chamber through microchannels relative to the total cell count.