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. 2024 May 9;2024(5):CD014715. doi: 10.1002/14651858.CD014715.pub2

Summary of findings 4. Accuracy of first‐ and second‐trimester ultrasound in detecting anomalies of individual organ systems and 46 selected structural anomalies.

Accuracy of first‐ and second‐trimester ultrasound in detecting anomalies of individual organ systems and 46 selected structural anomalies
  Two‐stage screening approach Single‐stage screening approach
First‐trimester scan
(detection < 14 weeks' gestation) First + second‐trimester scan
(total detection < 24 weeks' gestation)
Single second‐trimester scan
(detection < 24 weeks' gestation)
Studies Fetuses
(cases) Sensitivity
(95% CI)
Studies Fetuses
(cases)
Sensitivity
(95% CI)
Studies Fetuses
(cases)
Sensitivity
(95% CI)
Central nervous system
Overall (any type) 23 260,409
(843) 56.6
(47.6 to 65.2) 19 240,786
(779) 92.9
(85.9 to 96.6) 10 159,170
(663) 77.5
(65.5 to 86.2)
Anencephaly 30 548,041
(445) 99.5
(90.9 to 100) 24 317,464
(312) N/A*
Range: 0.0 (Taipale 2004) to 100 (remaining 23 studies)
23 1,284,752
(716)
95.6
(90.6 to 98.0)
Spina bifida 23 480,116
(286) 35.1
(22.0 to 51.1) 18 252,802
(147) 97.9
(90.0 to 99.6) 25 1,897,018
(946) 76.3
(65.7 to 84.4)
Holoprosencephaly 15 270,372
(99) 92.4
(71.6 to 98.3) 11 229,181
(90) N/A*
Range: 100 to 100
3 162,338
(13) N/A*
Range: 77.8 to 100
Hydrocephalus 14 253,796
(99) 4.2
(0.7 to 21.3) 10 218,872
(81) 75.4
(53.4 to 89.2) 8 61,812
(154) 76.0
(45.0 to 92.5)
Encephalocele 15 390,064
(84) 96.3
(62.8 to 99.8) 12 189,661
(47) N/A*
Range: 94.7 to 100 5 168,263
(37) 81.8
(49.7 to 95.4)
Respiratory
Overall (any type) 13 239,670
(158) 14.6
(9.7 to 21.6) 10 222,798
(146) 83.8
(72.2 to 91.2) 8 157,767
(63) 83.8
(67.1 to 92.9)
CDH 17 2,106,233
(520)
22.2
(10.1 to 42.2) 13 2,071,309
(513)
75.8
(44.7 to 92.4) 9 689,973
(243) 62.9
(56.7 to 68.7)
CPAM 7 207,768
(66) 1.4
(0.1 to 15.6) 5 195,687
(63) N/A*
Range: 92.9 to 100
7 352,922
(42) N/A*
Range: 50.0 to 100
Cardiac
Overall (any type) 28 2,719,925
(1543) 36.7
(27.7 to 46.6) 24 254,349
(1433) 81.5
(69.8 to 89.4) 17 800,716
(6917) 33.8
(24.7 to 44.2)
Septal defecta 34 301,645
(476) 32.7
(20.5 to 47.9) 28 275,487
(453) 73.3
(50.7 to 87.9) 11 1,056,215
(671) 36.7
(16.0 to 63.9)
Valvular anomaly (biventricular heart)b 17 247,073
(99) 26.8
(11.6 to 50.5) 14 229,673
(85) 99.7
(10.5 to 100) 10 1,056,169
(173) 38.6
(30.8 to 47.0)
Venous return anomalyc 6 74,351
(27) N/A*
Range: 0.0 to 100;
0.0 (Becker 2012; Kenkhuis 2018);
57.1 to 100 (remaining 4 studies)
4 66,388
(25) N/A*
Range: 0.0 to 100;
0.0 (Becker 2012; Kenkhuis 2018);
85.7 to 100 (Gireada 2022; Liao 2021) 2 723,265
(49) N/A*
Range: 25.0 (Ingale 2017) to 33.3 (van Velzen 2016)
Aortic arch anomalyd 18 242,677
(185) 24.8
(16.1 to 36.2) 13 219,782
(167) 69.4
(38.8 to 89.1) 8 1,047,246
(263)
31.6
(26.1 to 37.6)
Conotruncal anomalye 31 291,090
(284) 34.5
(21.1 to 50.9) 25 264,931
(255) 90.0
(76.5 to 96.2) 12 1,066,551
(606) 66.4
(38.8 to 86.1)
HRHSf 7 86,642
(25) 92.7
(0.0 to 100) 5 72,992
(23) N/A*
Range: 0.0 to 100;
0.0 (Gireada 2022);
100 (remaining 4 studies) 5 1,009,708
(40) 55.7
(35.3 to 74.4)
HLHSg 24 260,805
(155) 79.0
(45.2 to 94.5) 21 247,473
(147) 98.2
(75.8 to 99.9) 12 1,579,434
(392) 87.5
(79.1 to 92.8)
Other single ventricle defectsh 14 242,246
(67) 100
(58.8 to 100) 12 232,136
(63) N/A*
Range: 0 (Hernadi 1997) to 100 (remaining 11 studies) 7 1,328,100
(255)
85.6
(79.6 to 90.1)
Complex defects with atrial isomerismi 10 207,098
(28) N/A* 
Range: 0.0 to 100; 
0.0 (Vellamkondu 2017); 57.1 to 100 (remaining 9 studies) 8 203,174
(26) N/A*
Range: 91.7 to 100 4 1,011,866
(130) 75.1
(62.0 to 84.7)
Miscellaneous major cardiac defectsj 18 249,653
(166) 20.1
(2.1 to 75.1) 12 212,983
(74) 87.9
(69.6 to 95.8) 10 1,391,739
(978) 38.8
(23.3 to 56.9)
Minor cardiac defectsk 11 89,679
(198) 9.7
(2.9 to 27.8) 10 87,776
(181) 83.8
(52.8 to 96.0) 3 24,043
(78) N/A*
Range: 0 to 4.2
Thoracic and abdominal wall
Overall (any type) 17 251,923
(358) 95.6
(89.1 to 98.3) 14 235,051
(335) 99.0
(87.3 to 99.9) 7 58,014
(31) 90.8
(54.7 to 98.8)
Gastroschisis 17 276,233
(99) 100
(74.8 to 100) 13 241,309
(91) 100
(99.1 to 100) 10 811,149
(368) N/A*
Range: 0.0 to 100;
0.0 (Ingale 2017); 
93.5 to 100 (remaining 9 studies)
Omphalocele 23 288,522
(273) 98.0
(90.4 to 99.6) 18 250,335
(247) 99.2
(98.5 to 99.6) 9 827,508
(330) N/A*
Range: 79.3 to 100
Gastrointestinal
Overall (any type) 13 238,447
(155) 8.3
(4.9 to 13.8) 10 221,575
(138) 46.5
(39.0 to 54.1) 9 157,923
(197) 33.3
(14.8 to 59.0)
Oesophageal atresia 8 202,606
(26) N/A*
Range: 0.0 to 50.0;
0.0 (7 studies); 
50.0 (Iliescu 2013) 5 170,904
(18) N/A*
Range: 0.0 to 50.0;
0.0 to 0.25 (4 studies);
50.0 (Syngelaki 2019) 6 63,913
(19)
N/A*
Range: 0.0 to 100;
0.0 to 16.7 (5 studies);
100 (Drukker 2020)
Duodenal atresia 7 202,562
(23) N/A*
Range: 0.0 to 12.5 5 194,508
(21) 98.2
(0.0 to 100) 6 47,284
(45) 52.3
(18.2 to 84.4)
Small bowel obstruction 3 165,978
(18) N/A*
Range: 0.0 to 0.0 2 147,926
(12) N/A*
Range: 0.0 (Syngelaki 2019) to 33.3 (Liao 2021) 2 19,528
(3) N/A*
Range: 0.0 (Rydberg 2017) to 50.0 (Jacobsen 2011)
Urinary tract
Overall (any type) 19 253,523
(906) 23.5
(17.0 to 31.6) 16 236,649
(843) 87.1
(67.9 to 95.6) 9 158,415
(972) 60.6
(30.9 to 84.0)
Renal agenesis 15 210,855
(46) N/A*
Range: 0.0 to 100;
0.0 (10 studies);
15.3 to 50.0 (4 studies);
100 (Sainz 2020)
11 175,931
(39) 90.3
(45.5 to 99.1) 8 72,038
(31) N/A*
Range: 66.7 (Drukker 2020) to 100 (7 remaining studies)
  • Unilateral

7 74,458
(17) N/A*
Range: 0.0 (6 studies) to 100 (Brown 2021) 5 62,377
(15) 73.4
(38.0 to 92.5) 4 35,300
(16) N/A*
Range: 50.0 (Drukker 2020) to 100 (remaining 3 studies)
  • Bilateral

12 203,506
(30) 16.6
(6.5 to 36.2) 8 168,582
(24) 100
(57.8 to 100) 9 698,237
(103) 91.4
(68.9 to 98.1)
MCDK 15 238,157
(129) 6.8
(0.0 to 36.2) 13 230,144
(124) 80.3
(54.5 to 93.3) 5 38,486
(39) 82.1
(34.1 to 97.6)
  • Unilateral

6 153,637
(79) N/A*
Range: 0.0 (5 studies) to 100 (Whitlow 1999) 5 148,846
(78) 88.4
(79.6 to 93.7) 0
  • Bilateral

5 149,834
(10) N/A*
Range: 0.0 to 0.0 4 145,044
(9) N/A*
Range: 100 to 100 0
Other renal dysplasias 12 252,838
(87) 16.2
(9.9 to 25.4) 10 225,927
(77) N/A*
Range: 78.6 to 100 4 34,481
(18) 71.7
(16.9 to 96.9)
  • Unilateral

1 13,639
(2) N/A*
Range: 0.0 to 0.0 
(Grande 2012) 1 13,639
(2) N/A*
Range: 100 to 100
(Grande 2012) 2 23,216
(11) N/A*
Range: 20.0 (Hildebrand 2010) to 100 (Jacobsen 2011)
  • Bilateral

8 222,602
(40) 22.4
(11.5 to 39.3) 8 222,602
(40) N/A*
Range: 78.6 to 100 2 10,121
(2) N/A*
Range: 100 to 100
(Johnson 1997; Leiroz 2021)
Upper urinary tract obstruction 17 268,687
(225) 0.9
(0.0 to 10.8) 13 233.763
(188) 68.2
(38.7 to 87.9) 5 40,990
(85) 33.9
(2.3 to 91.8)
Lower urinary tract obstruction 18 262,135
(167) 94.0
(82.6 to 98.1) 16 250,018
(157) 99.0
(70.0 to 100) 7 194,823
(27) N/A*
Range: 83.3 to 100
Genital
Overall (any type) 8 177,556
(145) N/A*
Range: 0.0 to 0.0 6 169,543
(137) NA*
Range: 0.0 to 8.1 6 149,642
(107) N/A*
Range: 0.0 to 33.3;
0.0 to 3.0 (5 studies); 33.3 (Chen 2009)
Ambiguous genitalia 2 99,504
(6) N/A*
Range: 0.0 to 0.0
(Iliescu 2013; Syngelaki 2019) 1 94,713
(5) N/A*
Range: 80.0 to 80.0 (Syngelaki 2019) 1 13,516
(1) N/A*
Range: 0.0 to 0.0 (Drukker 2020)
Musculoskeletal
Overall (any type) 21 257,229
(696) 25.4
(18.2 to 34.3) 18 240,357
(635) 77.5
(59.9 to 88.8) 10 158,988
(536) 43.8
(29.3 to 59.5)
Skeletal dysplasias 16 269,236
(83) 54.1
(39.9 to 67.7) 14 246,393
(77) N/A*
Range: 50.0 to 100 8 670,352
(102) NA*
Range: 0.0 to 100;
0.0 (Jacobsen 2011);
50.0 to 100 (remaining 7 studies)
  • Lethal

10 229,873
(32) 56.5
(30.7 to 79.2) 9 224,819
(30) N/A*
Range: 100 to 100 4 189,617
(26) N/A*
Range: 75.0 to 100
  • Non‐lethal

7 146,455
(26) 37.2
(9.8 to 76.3) 5 123,612
(20) N/A*
Range: 0.0 to 100;
0 (Taipale 2004); 
50.0 to 100 (remaining 4 studies) 4 40,383
(9)
N/A*
Range: 0.0 to 66.7;
0.0 to 25.0 (3 studies); 
66.7 (Stefos 1999)
Limb reduction defect 18 273,864
(144)
62.0
(43.3 to 77.7) 13 233,886
(107) 100
(67.4 to 100) 9 211,781
(66) 46.3
(24.7 to 96.4)
Talipes 17 244,832
(266) 6.0
(2.2 to 15.6) 13 222,906
(241) 72.1
(46.4 to 88.5) 8 72,151
(133) 42.6
(24.0 to 63.5)
Ear, face and neck
Overall (any type) 16 247,420
(334) NA*
Range: 0.0 to 50.0;
0.0 to 22.2 (14 studies); 50.0 (Becker 2012; Iliescu 2013) 14 233,770
(321) 59.6
(37.8 to 78.1) 7 144,251
(117) 32.3
(14.8 to 56.7)
Orofacial clefts 19 276,776
(264) 13.5
(5.1 to 31.4) 15 241,811
(216) N/A*
Range: 0.0 to 100;
0.0 (Carvalho 2002; Hernadi 1997; Souka 2006);
57.1 to 100 (remaining 12 studies)
12 737,082
(786) 44.1
(26.9 to 62.9)
  • Cleft lip

9 181,324
(48) 0.7
(0.0 to 8.1) 8 176,533
(47) 68.4
(40.9 to 87.1) 5 109,843
(43) 76.7
(65.1 to 85.3)
  • Cleft lip and palate

8 176,319
(126) 31.7
(24.4 to 40.1) 6 162,669
(118) 100
(81.5 to 100) 8 324,854
(261) 81.9
(77.7 to 85.5)
  • Cleft palate

4 170,424
(26) N/A*
Range: 0.0 to 0.0 3 161,565
(23) 11.7
(1.8 to 49.4) 5 93,865
(34) 2.9
(0.1 to 53.4)
Multisystem anomalies
Overall (any type) 21 256,603
(413) 69.6
(59.0 to 78.5) 18 239,731
(386) 94.7
(85.2 to 98.2) 7 58,014
(73) 40.6
(4.5 to 90.9)
Fetal hydrops 20 252,415
(196) 72.4
(38.7 to 91.6) 18 240,293
(188) 96.6
(76.1 to 99.6) 3 26,856
(8) N/A*
Range: 0.0 to 100;
0.0 (Rydberg 2017); 80.0 (Drukker 2020); 100 (Lee 1998)
MCA or syndrome 28 298,117
(268) 70.4
(53.0 to 83.3) 23 259,930
(232) 96.0
(82.3 to 99.2) 9 301,085
(1119) 69.6
(24.9 to 94.0)
*N/A: meta‐analysis was not possible because of failure of the random‐effects models to converge due to a lack of variation between studies, or was not performed due to a lack of data (i.e. two or fewer studies available).
a‐kCardiac anomalies were grouped according to their anatomical classification as proposed by van Velzen 2016. 
Examples of anomalies that were included in each category:
aSeptal defects: e.g. major VSD, balanced AVSD.
bValvular anomalies (biventricular heart): e.g. significant pulmonary or aortic valve stenosis, Ebstein’s anomaly, significant tricuspid dysplasia or regurgitation.
cVenous return anomalies: e.g. total anomalous pulmonary venous return, partial anomalous pulmonary venous return.
dAortic arch anomalies: e.g. aortic coarctation, hypoplastic or interrupted aortic arch, double aortic arch, right aortic arch with left ductus arteriosus.
eConotruncal anomalies: e.g. Tetralogy of Fallot, double outlet right ventricle, transposition of the great arteries, truncus arteriosus, pulmonary atresia with VSD.
fHypoplastic right heart syndrome (HRHS): HRHS or hypoplastic right heart, pulmonary atresia with intact ventricular septum, critical pulmonary valve stenosis with right ventricular hypoplasia.
gHypoplastic left heart syndrome (HLHS): HLHS or hypoplastic left heart, aortic valve atresia or critical aortic valve stenosis with left ventricular hypoplasia.
hOther single ventricle defects: e.g. unbalanced AVSD, tricuspid atresia, pulmonary atresia without VSD, unspecified single ventricle defect.
iComplex defects with atrial isomerism: e.g. left or right atrial isomerism, heterotaxy syndromes.
jMiscellaneous major cardiac defects: e.g. unspecified major or complex heart defects, myocardial abnormalities, rhabdomyomas.
kMinor cardiac defects: e.g. minor or insignificant septal defects, minor or insignificant isolated valvular anomalies, isolated right aortic arch with right ductus arteriosus, isolated persistent left vena cava superior.
AVSD: atrioventricular septal defect; CI: confidence interval; CDH: congenital diaphragmatic hernia; CPAM: congenital pulmonary airway malformation; FN: false negative; FP: false positive; HRHS: hypoplastic right heart syndrome; HLHS: hypoplastic left heart syndrome; MCA: multiple congenital anomalies; MCDK: multicystic dysplastic kidney; TN: true negative; TP: true positive; VSD: ventricular septal defect

We did not determine specificity of ultrasound in detecting of anomalies of individual organ systems and of selected anomalies because all tests had near‐perfect specificity in the analyses, as well as in the subgroups of anomalies based on their severity (Table 1; Table 2; Table 3).