Fig. 8. Heatmap of theoretical PCR annealing temperature as a function of total concentration and strand ratio. Isolines show constant temperatures at integer degrees (light black lines). Theoretical minimum and maximum annealing temperatures during PCR are also shown (heavy black lines) with the current l-DNA implementation (white dot). The tinted region (right) shows the total concentrations and strand ratios where the fluorophore concentration is undetectable.
