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. 2024 May 9;24:355. doi: 10.1186/s12870-024-05064-x

Table 1.

Primers used for PCR amplification of jackfruit microsatellite loci [2]

Locus Primer sequence (5’-3’) EAS (bp) Ta/°C
MAA54a

F: AACCTCCAAACACTAGGACAAC

R: AGCTACTTCCAAAACGTGACA

211–239 59
MAA105

F: GTTGGGACACTGTGAACTATTC

R: AAAAGCTAGTGGATTAGATGCA

265–293 60
MAA122

F: CTGGCCTTCAGTTTTGTCAAC

R: CACCAGGCTTCAAGATGAAA

254–312 55
MAA140

F: CCATCCCCCATCTTTCCT

R: TCCTCGTTTGCCACAGTG

142–160 55
MAA145

F: CCAACGCATAGCCAAATC

R: AAATCCCAAACCCAACGT

275–299 50
MAA156

F: CTGGTGCTTCAGCCTAATG

R: TCAGCGTCAAAGATAACTCG

283–307 55
MAA178a

F: GATGGAGACACTTTGAACTAGC

R: CACCAGGGTTTAAGATGAAAC

250–258 55
MAA182

F: TACTGGGTCTGAAAAGATGTCT

R: CGTTTGCGTTTGGATAAAT

186–216 54
MAA196a

F: GGAATGTGGTAGATGAAACTCC

R: GACAAAAAAACAAAGGAAGAC

283–315 57
MAA196b

F: GAATGTGAGAGATAAATCTCC

R: GACAAAAAAACAAAGGAAGAC

337–377 46

EAS = expected allele size, Ta = annealing temperature